This Practice Note explains the procedural requirements and practical issues which need to be satisfied when dealing with witness evidence in criminal prosecutions in England and Wales. It covers the procedural requirements contained in the Criminal Procedure Rules (CrimPR), the sequence of questioning lay witnesses, examination-in-chief, refreshing the witness’s memory, dealing with previous inconsistent statements, hostile witnesses and the cross-examination and re-examination of witnesses. It also considers tendering a prosecution witness for cross-examination by the accused and an application for a witness summons in respect of a reluctant witness under section 2 of the Criminal Procedure (Attendance of Witnesses) Act 1965.